Composite

Part:BBa_K887072

Designed by: TSAI,LU-AN   Group: iGEM12_NCTU_Formosa   (2012-09-14)


Plac+B0034+zif268+alsS+B0034+PBSII+ilvC+B0034+HIVC+ilvD

This part contains three zinc fingers and following enzymes. These three enzymes will change pyruvate to 2-ketoisoalerate(the Intermediate of isobutanol). With additional zinc finger, these fusion protein can bind to the DNA program according to the order.These strategy can enhance productivity theoretically.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 6049
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3158
    Illegal AgeI site found at 4143
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2744
    Illegal BsaI site found at 5835
    Illegal BsaI site found at 6092
    Illegal BsaI.rc site found at 836
    Illegal BsaI.rc site found at 1430
    Illegal BsaI.rc site found at 3563


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Categories
Parameters
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